Apoptosis of human cervical cancer cell lines Caski induced by aspirin and its molecular mechanism

Objective To study the inhibiting effect and molecular mechanism of aspirin(ASA)on the proliferation of human cervical cancer cell lines Caski,so as to provide basis for its application in the clinical treatment of cervical cancer.Methods Caski cells were cultured in vivo and treated with ASA at the concentrations of 1.0,2.5,5.0,7.0,5.0,10.0 mmol/L.The cell proliferation inhibitory rates(CPIR)were tested by MTT method;The cell cycle and apoptosis were analyzed by flowcytometry(FCM);The apoptosis ladder of DNA was evaluated by agarose gel electrophoresis;The expression of apoptosis-related genes as Bcl-2,Bax and Caspase-3 et al were detected by Western blot method.Results ASA inhibited Caski cell proliferation in a time and dose dependent manner;After the treatment of ASA,the number of cells in G0/G1 and G2/M phases decreased significantly,and which in the population of S phases increased;The apoptosis rate increased significantly;The expression of Bcl-2 decreased,the expression of Bax and Caspase-3 increased.Conclusion ASA can inhibit the proliferation of Caski cell in vivo,the possible mechanism is inhibiting the DNA synthesis,changing the cell cycle phase and inducing the apoptosis.