2013•Anhui Medical and Pharmaceutical JournalRequires access

Determination of baicalin in Angong Niuhuang Pill by HPLC

Gao Qing

Abstract

Objective To develop an HPLC method for the quantitative determination of baicalin in Angong Niuhuang Pill.Methods The chromatographic conditions included column Hypersil C18(250 mm×4.6 mm,5 μm),with mobile phase of acetonitrile-water-phosphoric acid(28:72:0.2).The flow rate was set at 1.0mL·min-1,wavelength at 280 nm.Results The linearity of baicalin was in the sample range of 100-1 200 ng,r=0.999 8 and the average recovery was 101.6% with RSD 2.3%.Conclusion This method is simple with good reproducibility and available for quality control of baicalin in Angong Niuhuang Pill.

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Key concepts: Baicalin, Chromatography, High-performance liquid chromatography, Phosphoric acid, Reproducibility, Chemistry, Pill, Medicine