Protective effect of bipinnatpolyacetylenicloside on acute liver injury induced by CCl_4 in mice

Objective To study the protective effect of bipinnatpolyacetylenicloside on acute liver injury induced by CCl4 in mice.Methods Mice were divided into 6 groups including control group,model group,positive group Silymarin,and bipinnatpolyacetylenicloside treatment(12.5,25,50 mg·kg-1) group.Acute hepatic injury was induced by injecting 0.1ml(10g)-1 0.2% olive oil solution of carbon tetrachloride and mice in bipinnatpolyacetylenicloside treatment group were intragastric administered with bipinnatpolyacetylenicloside for 6 days before given carbon tetrachloride.Serum AST and ALT were detemined.The contents of SOD,GSH-Px,MDA,and NO in liver homogenate were determined.The index as well as the histopathological test of liver was observed and the expression of NF-κB p65 in liver was tested by immunohistochemistry.Results Compared with those in model group,the levels of AST,ALT in serum,the contents of MDA,NO in liver homogenate were reduced significantly while the levels of SOD,GSH-Px in liver homogenate were markedly increased.In bipinnatpolyacetylenicloside treatment(12.5,25,50 mg·kg-1) groups,histopathological study showed that the degenerative and necrotic liver cells could be evidently ameliorated,and,immunohistochemistry research demonstrated that the expression of NF-κB was dramatically decreased.Conclusion The bipinnatpolyacetylenicloside has a protective effect on the carbon tetrachloride induced acute liver injury in mice,the mechanism of which may be related to its antioxidant characteristic as well as the production of NO and the expression of NF-κB being inhibited.