Optimization of ultrasonicextraction of flavonoids from Potentilla discolor Bge. using response surface methodology

Response surface methodology(RSM) was used for optimizing ultrasonic extraction conditions in the extraction of flavonoids from Potentilla discolor Bge.Effects of ethanol concentration,ultrasonic power,extraction time and ratio of solvent to material on flavonoids yield were analyzed with response surface methodology.The results show that regression equation fit well with experimental data,the optimum procedure of flavonoids extraction are:ethanol concentration of 90%,ultrasonic power of 720 W,extraction time of 35 min,ratio of solvent to material of 30 mL/g and that the flavonoids yield is up to 2.86%.