ScholarLens Back to search
2012•Journal of Northeast Normal UniversityRequires access

Synthetic extraction of RNA and β-(1,3)-D-Glucan from waste beer yeast

Guirong Zhang

Open publisher page 0 citations

Abstract

The present paper studies the influence of temperature and NaOH concentration on the synthetic extraction of RNA and β-(1,3)-D-Glucan from waste beer yeast.The experiment uses the alkali-enzyme extraction method and optimizes the dilute alkali extraction conditions to bring about the synthetic extraction.The experiment shows that the extraction rate is 7.65% and the degree of purity is 70.24% with a temperature of 100° C and the concentration of NaOH for 4%.Furthermore,the experiment investigates the influence of neutral protease and alkaline protease on the extraction of β-(1,3)-D-Glucan and finds that neutral protease may facilitate more β-(1,3)-D-Glucan of higher purity.It is believed that the experiment results lay a foundation for industrial production.

About this research paper

What this paper is about

The present paper studies the influence of temperature and NaOH concentration on the synthetic extraction of RNA and β-(1,3)-D-Glucan from waste beer yeast.The experiment uses the alkali-enzyme extraction method and optimizes the dilute alkali extraction conditions to bring about the synthetic extraction.The experiment shows that the extraction rate is 7.65% and the degree of purity is 70.24% with a temperature of 100° C and the concentration of NaOH for 4%.Furthermore,the experiment investigates the influence of neutral protease and alkaline protease on the extraction of β-(1,3)-D-Glucan and finds that neutral protease may facilitate more β-(1,3)-D-Glucan of higher purity.It is believed that the experiment results lay a foundation for industrial production.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The present paper studies the influence of temperature and NaOH concentration on the synthetic extraction of RNA and β-(1,3)-D-Glucan from waste beer yeast.The experiment uses the alkali-enzyme extraction method and optimizes the dilute alkali extraction conditions to bring about the synthetic extraction.The experiment shows that the extraction rate is 7.65% and the degree of purity is 70.24% with a temperature of 100° C and the concentration of NaOH for 4%.Furthermore,the experiment investigates the influence of neutral protease and alkaline protease on the extraction of β-(1,3)-D-Glucan and finds that neutral protease may facilitate more β-(1,3)-D-Glucan of higher purity.It is believed that the experiment results lay a foundation for industrial production.

Key concepts: Extraction (chemistry), Protease, Yeast, Glucan, Alkali metal, Chemistry, Chromatography, Enzyme

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Synthetic extraction of RNA and β-(1,3)-D-Glucan from waste beer yeast — Research Paper | ScholarLens