Demulsified Dispersive Liquid-Liquid Microextraction Coupled with High Performance Liquid Chromatography for the Determination of 2,4-dichlorophenol and 2,6-dichlorophenol in Environmental Water Samples

A rapid, simple and sensitive method, the demulsified dispersive liquid-liquid microextraction was established,and coupled with high performance liquid chromatography, which was successfully used for the determination of 2,4-dichlorophenol(2,4-DCP) and 2,6-dichlorophenol(2,6-DCP) in environmental water samples. The extraction conditions such as the type and volume of extraction solvent and dispersive/demulsified solvent, salt concentration, p H and other factors were optimized, the best demulsified dispersive liquid-liquid microextraction conditions were found. Through the determination of2,4-DCP and 2,6-DCP, the linear range of 2,4-DCP and 2,6-DCP were 5~600 μg/L(r=0.999 8)and 5~600 μg/L(r=0.999 7)respectively. The limits of detection(LOD)(3σ) were 2.06 μg/L and 2.88 μg/L. To determine the standard solution of 200 μg/L, the relative standard deviation(RSD) were 1.71% and 2.67%(n =6)respectively. The developed method was successfully applied to the determination of trace amount of 2,4-DCP and 2,6-DCP in three kinds of real environmental water samples,with the spiked recoveries in the range of 83.2%~118.2% and RSD of 0.56%~17.81%(n=3).