Study on Rapid Propagation Techniques of Impatiens Hawkerii in Vitro Culture

The new stem nodes of Impatiens hawkerii with terminal buds and axillary buds were taken as explant cultured in vitro to induce buds sprouting and multiplication. Media supplemented with different plant hormones and concentration of them were required for different stage of the culture. The medium for germination: MS+6 BA2.0mg/L+NAA0.2mg/L. Terminal buds of the explants sprouted early and grew fast in this medium. The medium for subculture and shoot multiplications: MS+6 BA1.5mg/L+NAA0.2mg/L, which was the first option. The medium for rooting:1/2MS+NAA0.2mg/L. The rooting rate was 100% and the quality of the roots grew well. The survival rate of the plantlet was 95% after transplanting under suitable condition.