Mechanism of Reabsorption of Yiqihuayu Decoction on Lumbar Intervertebral Disc Herniation of Rupture Type

Objective:To detect the mechanism of reabsorption of Yiqihuayu decoction on lumbar intervertebral disc herniation of rupture type.Methods:All 28 male SD rats were randomly divided into normal control group,model group,Yiqihhuayu decoction group,and azathioprim group,7 rats in each group.The successful animal models were killed 20 days after administration.The immunohistochemistry was used to detect CD19 of the lumbar disc,and the flow cytometry was used to examine the levels of T cells(CD3,CD4,and CD8).Results: The rate of positive expression of B cells(CD19) in Yiqihuayu decoction group was significantly higher than the model group and azathioprine group(P0.05).There was positive expression of T cells(CD3,CD4,and CD8) in each group,and the levels of positive expression in Yiqihuayu decoction group and model group were both higher than azathioprine group.Conclusion:The disc reabsorption and diminution of the lumbar intervertebral disc herniation is relevant with the autoimmune response.Yiqihuayu decoction can attract activity T and B cells,thus to promote the role of autoimmune response,then to promote disc reabsorption of lumbar intervertebral disc herniation.