Optimization of the extraction technology of Wuhua Yin by orthogonal design

Objective To optimize the extraction technology of Wuhua Yin.Methods Orthogonal experiment was adopted to optimize the extraction technology by using the volatile oil extraction rate of Lv Emei as an index to determine the process for volatile oil,the quercetin and magnolol extraction rate as an index to screening the ethanol extraction process,total flavonoids extraction rate as an index to determine the water extraction process.Results The optimal process of volatile oil extraction was as follows: add 10 times of water,soak for 2 hours,and then extract for 9 hours.The optimal process of ethanol extraction was as follows: add 10 times of 85% ethanol,and then extract for 2 hours.The optimal process of water extraction was as follows: add 8 times of water,and reflux extraction for 3 times,1 hour for each time.Conclusion The optimized extraction process is stable and feasible.