HPLC Determination of Content of Ranitidine Hydrochloride Capsules

Objective To establish HPLC method to determine the content of ranitidine hydrochloride capsules.Methods The column was packed with octadecylsilane bonded silica gel(4.6 mm×150 mm,5 μm).The mobile phase was consisted of a mixture of phosphate buffer and acetonitrile(pH7.1).Acetonitrile was used for gradient elution at a flow rate of 1.5 ml/min.Detection wavelength was 230 nm.The column temperature was 35℃.Results The calibration curve of ranitidine hydrochloride was linear within the range of 0.053 67～0.178 9 mg/ml(r= 0.999 9).The mean recovery was 99.5%.Conclusion The method is specific with good reproducibility and wide linear range.It can be applied for the quality control of ranitidine hydrochloride capsules.