ScholarLens Back to search
2015•The Pharma InnovationOpen access

RP-HPLC method Development and Validation studies of Ranitidine Hydrochloride and Domperidone in Tablets

Krishnasis Chakraborty, G Mubeen, N Lalitha, Ritu Kimbahune

Open full text 6 citations

Abstract

This study proposes a liquid chromatography (RP-HPLC) method for quantitative analysis of the most widely prescribed combinations of Ranitidine hydrochloride, Domperidone and Magaldrate in tablet dosage form in the presence of Metronidazole as an internal standard. The chromatography was done on a C18 column (250 mm × 4.6 mm, 5 (m) as stationary phase and Methanol: Potassium dihydrogen o-phosphate (65:35 % v/v, pH 3.0 adjusted to 1% OPA) as mobile phase at a current rate of 1mL/min and measured at 227nm. Retention time was found to be 2.68min, 3.66min and 5.25min for RH, DM and MZ respectively. The assay of RH and DM in the formulation was found to be 100.33% w/w and 99.53% w/w respectively. The linearity range for RH was 3-150 µg/mL and for DM was 0.2-10 µg/mL. The developed method was validated in accordance to ICH guidelines.

Open-access reader

About this research paper

What this paper is about

This study proposes a liquid chromatography (RP-HPLC) method for quantitative analysis of the most widely prescribed combinations of Ranitidine hydrochloride, Domperidone and Magaldrate in tablet dosage form in the presence of Metronidazole as an internal standard. The chromatography was done on a C18 column (250 mm × 4.6 mm, 5 (m) as stationary phase and Methanol: Potassium dihydrogen o-phosphate (65:35 % v/v, pH 3.0 adjusted to 1% OPA) as mobile phase at a current rate of 1mL/min and measured at 227nm. Retention time was found to be 2.68min, 3.66min and 5.25min for RH, DM and MZ respectively. The assay of RH and DM in the formulation was found to be 100.33% w/w and 99.53% w/w respectively. The linearity range for RH was 3-150 µg/mL and for DM was 0.2-10 µg/mL. The developed method was validated in accordance to ICH guidelines.

Why it matters

OpenAlex reports 6 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

This study proposes a liquid chromatography (RP-HPLC) method for quantitative analysis of the most widely prescribed combinations of Ranitidine hydrochloride, Domperidone and Magaldrate in tablet dosage form in the presence of Metronidazole as an internal standard. The chromatography was done on a C18 column (250 mm × 4.6 mm, 5 (m) as stationary phase and Methanol: Potassium dihydrogen o-phosphate (65:35 % v/v, pH 3.0 adjusted to 1% OPA) as mobile phase at a current rate of 1mL/min and measured at 227nm. Retention time was found to be 2.68min, 3.66min and 5.25min for RH, DM and MZ respectively. The assay of RH and DM in the formulation was found to be 100.33% w/w and 99.53% w/w respectively. The linearity range for RH was 3-150 µg/mL and for DM was 0.2-10 µg/mL. The developed method was validated in accordance to ICH guidelines.

Key concepts: Domperidone, Chromatography, Ranitidine, High-performance liquid chromatography, Chemistry, Potassium, Methanol, Retention time

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
RP-HPLC method Development and Validation studies of Ranitidine Hydrochloride and Domperidone in Tablets — Research Paper | ScholarLens