Epigallocatechin-3-gall-ate on Colorectal Cancer Cells in vitro

ObjectiveTo study the effect of epigallocatechin-3-gallate(EGCG) on the proliferation of colorectal cancer cells(LoVo cells 、SW480 cells、HT29cells andHCT-8cells) cell cycle and cell apoptosis. MethodsColorectal cancer cells were cultured in vitro and the proliferation ability of cultured cells in different concentration of EGCG(10,20,35 μg/ml)were tested by MTT analysis,the cell apoptosis and cell cycle were detected with flow cytometry. ResultsThe inhibition of EGCG on colorectal cancer cells showed dose dependence,but showed no time dependence except HT29 cells.EGCG could enhance apoptisis rate of colorectal cancer cells.At the mean time,EGCG could block the cell cycle of SW480 cells and LoVo cells at G0/G1 phase,hind their conversion to S phase;could block HCT-8 cell at G2/M phase,hind their conversion to M phase;could block HT29 cell at S phase,hind their conversion to G2 phase and inhibit their proliferation. ConclusionEGCG can significantly inhibit the proliferation of colorectal cancer cells by changing cell cycle and inducing cell apoptosis.The mechanism may be related to the inhibition to certain gene and signal transduction system.