Association between the matrix metalloproteinase - 12 gene polymorphism and susceptibility of non - small cell lung cancer in northern China

Objective:To investigate the association between matrix metalloproteinases-12(MMP-12-82A/G SNP) polymorphism and susceptibiliy of non-small cell lung cancer(NSCLC) in northern China.Methods: This hospital-based case-control study included 300 patients with non-small cell lung cancer(161 squamous carcinoma and 139 adenocarcinoma) and 300 healthy controls.Total DNA was extracted form peripheral blood leucocyte using proteinase K digestion followed by a salting out procedure.Polymorphisms of MMP-12 gene were analyzed by PCR-restriction fragment length polymorphism(RFLP).Results: The percentage of smokers in the patients(62.0%) was significantly higher than that in healthy controls(36.3%,P0.01).Smoking may increase the risk of NSCLC(age and gender adjusted OR=3.74,95% CI=2.451-5.705).The distribution of MMP-12-82 A/G SNP in the promoter region of healthy controls was not significantly different from the patients with NSCLC evaluated by Hardy-Weinberg equilibrium(P0.05).The allelotype and genotype distribution of the MMP-12-82A/G SNP was not significantly different between NSCLC patients and healthy controls according to smoking status and histological type(P0.05).Conclusion: Smoking increases the risk of developing NSCLC.The allelotype and genotype distribution of MMP-12-82A/G SNP in the NSCLC patients is not significantly different from that in healthy controls.