Effect of exogenous phosphocreatine on exhaustive exercise mice

Objective:To observe the influence of exogenous phosphocreatine on exhaustive exercise mice,and research the mechanism of antifatigue.Methods:The method of mice weight-loading swimming was used to set up the model of exhaustive exercise.The mice were divided into group of exhaustion with medicine as group A,group of exhaustion without medicine as group B,group of swimming for 8 min without medicine as group C,and group of swimming for 8 min with medicine as group D.Twenty min before the experiment,mice in the groups A and D were intraperitoneally injected with creatine phosphate sodium by the standard of 1000mg/kg,and those in the groups B and C were intraperitoneally injected with the same volume of normal saline.The exhaustive swimming experiment was conducted on the loading weight with 6% of the weight of each mouse.Serum SOD activity and MDA content were determined by using biochemistry colorimetry,and the exhaustive time was recorded.Results:The SOD activity in groups A,C and D was stronger than in group B(P0.05,0.01),and stronger in group D than in group A(P0.05).The MDA content in group B was higher than in group C(P0.01),and higher in group A than in group D(P0.05).The time from swimming to exhaustion in group B was significantly shorter than in group A(P0.05).Conclusion:The mechanism by which exogenous phosphocreatine strengthens the ability of organisms to fight against fatigue may be related to the enhancement of SOD activity and reduction of MDA content,which improves the organism's ability to resist fatigue by enhancing the removal of the free radicals.