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2004•Journal of Qilu OncologyRequires access

Screening and cloning of gastric carcinoma-related gene by mRNA differential display

You‐Wei Zheng

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Abstract

OBJECTIVE:To isolate and clone gastric carcinoma related gene. METHODS:The specially expressed cDNA fragments of gastric carcinoma were screened by differential display polymerase chain reaction (DD-PCR) after extracting the total RNA from tissues of human gastric carcinoma and normal gastric mucosa, then cloned these fragments to plasmid TA,and subsequently underwent sequence analysis,homogenous comparing and RNA dot hybridization.RESULTS:Obvious differences in gene expression between gastric carcinoma and normal gastric mucosa were found in 16 fragments express,of which 9 fragments were overexpressed and 7 fragments default expressed in gastric carcinoma.Among them 4 fragments were cloned and by sequence analysis,homogenous comparing and dot hybridization,one of them showed novel .CONCLUSION:The specially expressed EST sequence might be the gastric carcinoma related oncogene and maybe play a certain role in the development of gastric carcinoma.

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OBJECTIVE:To isolate and clone gastric carcinoma related gene. METHODS:The specially expressed cDNA fragments of gastric carcinoma were screened by differential display polymerase chain reaction (DD-PCR) after extracting the total RNA from tissues of human gastric carcinoma and normal gastric mucosa, then cloned these fragments to plasmid TA,and subsequently underwent sequence analysis,homogenous comparing and RNA dot hybridization.RESULTS:Obvious differences in gene expression between gastric carcinoma and normal gastric mucosa were found in 16 fragments express,of which 9 fragments were overexpressed and 7 fragments default expressed in gastric carcinoma.Among them 4 fragments were cloned and by sequence analysis,homogenous comparing and dot hybridization,one of them showed novel .CONCLUSION:The specially expressed EST sequence might be the gastric carcinoma related oncogene and maybe play a certain role in the development of gastric carcinoma.

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Available abstract

OBJECTIVE:To isolate and clone gastric carcinoma related gene. METHODS:The specially expressed cDNA fragments of gastric carcinoma were screened by differential display polymerase chain reaction (DD-PCR) after extracting the total RNA from tissues of human gastric carcinoma and normal gastric mucosa, then cloned these fragments to plasmid TA,and subsequently underwent sequence analysis,homogenous comparing and RNA dot hybridization.RESULTS:Obvious differences in gene expression between gastric carcinoma and normal gastric mucosa were found in 16 fragments express,of which 9 fragments were overexpressed and 7 fragments default expressed in gastric carcinoma.Among them 4 fragments were cloned and by sequence analysis,homogenous comparing and dot hybridization,one of them showed novel .CONCLUSION:The specially expressed EST sequence might be the gastric carcinoma related oncogene and maybe play a certain role in the development of gastric carcinoma.

Key concepts: Molecular biology, Complementary DNA, clone (Java method), Biology, Gene, Gastric carcinoma, Carcinoma, Differential display

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