2004•PubMedRequires access
[Isolation of differentially expressed cDNA sequences in human gastric carcinoma by cDNA microarray].
Hailong Xie, Xiao-Jun Zhou, Jie-Yu Chen, Wenbin Huang, Lihua Zhang, Fangqiu Li
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Abstract
BACKGROUND & OBJECTIVE: Some gastric carcinoma-related genes haven't been identified yet. The study was designed to isolate and identify differentially expressed cDNA sequences in gastric carcinoma, and further clone gastric carcinoma-related genes. METHODS: The differences of gene expression profile between 30 gastric carcinoma tissues and their adjacent normal tissues were analyzed by cDNA microarray which representing about 4 892 cDNA sequences, the differentially expressed cDNA sequences were analyzed by bioinformatics, and selected cDNA sequences were confirmed by reverse transcripase-polymerase chain reaction (RT-PCR). RESULTS: A total of 33 differentially expressed cDNA sequences were identified in gastric carcinoma, among which 18 up-regulated in gastric carcinoma,while 15 down-regulated. MDSCBC11 clone,represented a novel gene, located in chromosome band 1p35-36, and significantly down-regulated in 13 of 30 (43%) gastric carcinomas. CONCLUSIONS: Some gastric carcinoma-related cDNA sequences have been identified,they might be involved in pathogenesis of gastric carcinoma. This study provides a useful clue for further clone gastric carcinoma-related genes.