Screening of colorectal carcinoma-related gene by mRNA differential display

Objective To isolate and clone colorectal carcinoma-related gene. Methods The total RNA was extracted from the tissues of human colorectal carcinoma and normal colorectal mucosa, and the specifically expressed cDNA fragments of colorectal carcinoma were screened by way of differential display polymerase chain reaction (DD-PCR). The cDNA fragments yielded from the above procedures were cloned into the plasmid TA, and subsequently underwent sequencing analysis and dot blot hybridization. Results Two novel cDNA fragments were obtained which were highly expressed in the colorectal carcinoma tissues but not in normal colorectal mucosa. Conclusion These 2 specifically expressed cDNA fragments isolated by DD-PCR might be the colorectal carcinoma-related oncogenes in quest.