Expression and significance of P16~(INK4a) and P14~(ARF) proteins in laryngeal squamous cell carcinoma

Objective To study the expression and clinical significance of P16INK4a and P14ARF proteins in laryngeal squamous cell carcinoma. Methods The expression of P16INK4a and P14ARF proteins was detected in 71 carcinoma samples by immunohistochemistry (EnVision method). Results The negative expression of P16INK4a and P14ARF was detected in 35 samples(49.3 %)and 29 samples(40.8 %) respectively. While 15 cases(21.1 %)showed simultaneous loss in both proteins. Rank correlation analysis revealed that there was no correlation between negative expression of P16INK4a and P14ARF proteins in carcinoma(P0.05). The negative expression rate of P16INK4a or P14ARF was unrelated to the clinical data including gender,age and clinical stage. The overpositive proportion of all P14ARF positive cases in stage I to II was 41.7% and 11.1% in stage III to IV. Conclusions The aberrance of P16INK4a and P14ARF gene might be associated with the oncogenesis of carcinoma. There was no correlation between negative expression of P16INK4a and P14ARF proteins in carcinoma. Moreover,the loss of P14ARF protein occurred in the early stage of carcinoma.