Expression of p14~(ARF) and E2F-1 protein in human squamous cell carcinoma of lung and their pathological significance

Objective To detect the expression of p14ARF and E2F-1 in human squamous cell carcinoma of lung and explore their role in the development of human squamous cell carcinoma(SCC)of lung and clinicopathological significance.Methods Thirteen cases of inflammation of the lung(the control group)and 56 cases SCC of lung were enrolled in this study.All specimens were affirmed by pathology.p14ARF protein and E2F-1 protein were detected by immunohistochemical examination of paraffin-embedded sections.Results ①The positive rate of p14ARF protein in SCC and the control group were 41.1% and 76.9%,respectively,the difference was significant(χ2=5.435,P=0.020).②The positive rate of E2F-1 in SCC was 55.4%,which was significantly higher than 7.7% in control group(χ2=9.639,P=0.002).Expression of E2F-1 in SCC was closely related to tumor stage,age and lymph node metastasis(P0.05).③There was an inverse correlation between p14ARF protein and E2F-1 protein(rs=-0.359,P0.05).Conclusion The down-regulation of p14ARF expression and up-regulation of E2F-1 expression may contribute to the carcinogenesis and development of SCC of lung and can be a biological marker for diagnosis and judging malignancy degree of SCC of lung.