Effects of Plant Hormone on Callus Induction and Bud Differentiation and Proliferation of Aloe aristata Haw

The research aimed to provide theoretical basis for the establishment of the rapid propagation system of Aloe aristata Haw.With tender stems and leaves of A.aristata as explants,the effects of different concentrations and proportions of plant hormone on its callus induction and bud differentiation and proliferation were discussed through orthogonal test.2,4-D was a main influencing factor inducing the callus of A.aristata and the induction effect was best at its concentration of 1.5 mg/L.The optimum hormone combination for the formation of A.aristata callus was 1.5 mg/L 2,4-D +0.6 mg/L 6BA + 0.3 mg/L NAA + 0.1 mg/L KT and the callus presented flavovirens and loose with the formation rate reaching 100%.The optimum hormone combination for the bud differentiation was 1.2 mg/L 6-BA + 0.1 mg/L NAA.And properly adding the concentration of 6-BA could increase the germination rate of callus.The water content of medium had certain effects on callus formation and the germination.This research provided an effective approach for the rapid propagation in vitro and industrialization production of Aloe aristata Haw.