A Preliminary Study on Callus Induction of Aloe barbadensis

The influence upon callus induction and its growth by the category and size of the explants, method of implanting, combination of plant growth regulators, and the effect of AgNO_3 and vitamin C was studied when the segments of stem, shoot, leaf and root of Aloe barbadensis were applied as explants, MS was adopted as elementary medium added with varied quantities of 2,4-D, NAA, KT and the different combinations of these plant growth regulators, and different contents of AgNO_3 and Vitamin C were added into the medium. It was indicated by the results that the best explants were 2 cm long stems, the best implanting method was the horizontal placement, the best combination of plant growth regulators was NAA 15 mg/L +2,4-D 4 mg/L +KT 0.1 mg/L, with which the callus induction rate reached to be 90%. It was also showed that the addition of 10 mg/L AgNO_3 could promote the growth of the callus, and 5 mg/L of vitamin C was helpful to inhibit callus browning in the culture process.