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2006•Unpublished venueRequires access

Extraction of Plant Esterase by Polyethylene Glycol Ammonium Sulfate Aqueous Two-phase System

Meng Ling, Dan Yang

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Abstract

The paper discussed the distribution of plant esterase in the PEG/(NH_4)_2SO_4 two-phase system.The dependence of partition coefficient of plant esterase and total protein,and the volume ratio of the top phase to the bottom phase on the percentage of PEG and(NH_4)_2SO_4,pH were studied respectively,and the appropriate separation conditions were determined.The suitable extraction conditions are 27 % PEG1000,13 %(NH_4)_2SO_4,pH 7.5.It was shown that the partition coefficient of K_(e) of (10.246 2),K_(p) of 2.316 0,the yield of 94.8 %.

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What this paper is about

The paper discussed the distribution of plant esterase in the PEG/(NH_4)_2SO_4 two-phase system.The dependence of partition coefficient of plant esterase and total protein,and the volume ratio of the top phase to the bottom phase on the percentage of PEG and(NH_4)_2SO_4,pH were studied respectively,and the appropriate separation conditions were determined.The suitable extraction conditions are 27 % PEG1000,13 %(NH_4)_2SO_4,pH 7.5.It was shown that the partition coefficient of K_(e) of (10.246 2),K_(p) of 2.316 0,the yield of 94.8 %.

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Available abstract

The paper discussed the distribution of plant esterase in the PEG/(NH_4)_2SO_4 two-phase system.The dependence of partition coefficient of plant esterase and total protein,and the volume ratio of the top phase to the bottom phase on the percentage of PEG and(NH_4)_2SO_4,pH were studied respectively,and the appropriate separation conditions were determined.The suitable extraction conditions are 27 % PEG1000,13 %(NH_4)_2SO_4,pH 7.5.It was shown that the partition coefficient of K_(e) of (10.246 2),K_(p) of 2.316 0,the yield of 94.8 %.

Key concepts: Ammonium sulfate, Partition coefficient, Polyethylene glycol, PEG ratio, Extraction (chemistry), Esterase, Chemistry, Chromatography

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