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Pectinase-Extraction with PEG/(NH_4)_2 SO_4 Two-Phase Aqueous System

Xu Wang

Abstract

The extraction behavior of pectinase in two-phase aqueous system was investigated. The effects of the molecular weight and concentration of polyethylene glycol, pH, the concentrations of NaCl and extract temperature on the extraction coefficient K were studied. The results showed that the optimum conditions are as follows: the mass composition in two-phase aqueous system PEG1000 27%, (NH4)2SO4 19%, NaCl 0.002%, pH5.0, the highest extraction coefficient K obtained in experiments can reach 14.

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Key concepts: Polyethylene glycol, Pectinase, Extraction (chemistry), PEG ratio, Aqueous solution, Chemistry, Aqueous two-phase system, Chromatography