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Extraction of esterase with PEG/NaH_2PO_4 two-phase aqueous system

Jiang Bin, Feng Zhi-biao, Yi Chen

Abstract

The extraction of esterase in two-phase aqueous system was investigated.The effects of the molecular weight and concentration of polyethyleneglycol,pH,the concentrations of NaCl and extract temperature on the extraction coefficient K were studied.The results showed that the optimum conditions were as follows:the mass composition in two-phase aqueous system 23%PEG1000(w/w),16%NaH2PO4(w/w),pH=4,the highest extraction coefficient K could reach 4.33,the purification fold was 2.85 and the yield of plant esterase could reach 95%.

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Available abstract

Key concepts: Extraction (chemistry), Aqueous solution, Chromatography, Esterase, PEG ratio, Chemistry, Yield (engineering), Aqueous two-phase system