Selection of optimum extraction process for Kanlisha by orthogonal design

Objective To find out the optimum extraction process for Kanlisha(traditional Chinese medicines).Methods The yield of extracts and the contents of cimicifugoside,chuanxiongzine and ferulic acid were taken as the assessment index,and HPLC was used for simultaneous determination of three effective ingredients.Different extraction processes with different solvents(water,vinegar,ethanol) were compared,and the third process was optimized with orthogonal design.Results The optimum extraction process is:Adding 8 times amount of 80%(V∶V) ethanol,and extracting for two times,1 h each time.Conclusions The optimum extraction process is reasonable,stable and fit for a large production.