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HPLC-RI determination of four ginkgolides in Ginkgo biloba extracts

Guiqing Kai

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Abstract

Objective:To establish an HPLC method for the determination of four ginkgolides [ ginkgolide A (GA), ginkgolide B (GB),ginkgolide C (GC),and hilobalide (BB) ] in Ginkgo biloba extracts (GBE).Methods:The Sym- metryshield C_(18) (3.9 mm×150 mm,5 μm)column was used,the mobile phase consisted of methanol-water(30: 70) at a flow rate of 1.0 mL·min~(-1),the detection was carried out with refractive index detector.Four ginkgolides were measured with the external standard method.Results:The linear ranges of four ginkgolides were O.02-0.24 mg·mL~(-1) (correlation coefficients were between 0.9984-0.9998);The limits were between 0.025-0.035 μg; Recoveries were 99.0%-100.2%,respectively.Conclusion:The method is simple,accurate and sensitive.It is suitable for the routine quality control.

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Objective:To establish an HPLC method for the determination of four ginkgolides [ ginkgolide A (GA), ginkgolide B (GB),ginkgolide C (GC),and hilobalide (BB) ] in Ginkgo biloba extracts (GBE).Methods:The Sym- metryshield C_(18) (3.9 mm×150 mm,5 μm)column was used,the mobile phase consisted of methanol-water(30: 70) at a flow rate of 1.0 mL·min~(-1),the detection was carried out with refractive index detector.Four ginkgolides were measured with the external standard method.Results:The linear ranges of four ginkgolides were O.02-0.24 mg·mL~(-1) (correlation coefficients were between 0.9984-0.9998);The limits were between 0.025-0.035 μg; Recoveries were 99.0%-100.2%,respectively.Conclusion:The method is simple,accurate and sensitive.It is suitable for the routine quality control.

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Available abstract

Objective:To establish an HPLC method for the determination of four ginkgolides [ ginkgolide A (GA), ginkgolide B (GB),ginkgolide C (GC),and hilobalide (BB) ] in Ginkgo biloba extracts (GBE).Methods:The Sym- metryshield C_(18) (3.9 mm×150 mm,5 μm)column was used,the mobile phase consisted of methanol-water(30: 70) at a flow rate of 1.0 mL·min~(-1),the detection was carried out with refractive index detector.Four ginkgolides were measured with the external standard method.Results:The linear ranges of four ginkgolides were O.02-0.24 mg·mL~(-1) (correlation coefficients were between 0.9984-0.9998);The limits were between 0.025-0.035 μg; Recoveries were 99.0%-100.2%,respectively.Conclusion:The method is simple,accurate and sensitive.It is suitable for the routine quality control.

Key concepts: Ginkgolides, Chemistry, Ginkgo biloba, Chromatography, High-performance liquid chromatography, Ginkgo, Ginkgoales, Pharmacognosy

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