The Extraction of Polysaccharide from White Wax Scale and Analysis of Monosaccharide Compositions

White Wax Scale(Ericerus pela Chavannes) polysaccharide was extracted with water,and precipitated by alcohol.The contents of polysaccharide、protein and amino acids were measured.The capillary zone electrophoresis(CZE) method was developed to separate and identify the monosaccharide composition of white wax scale polysaccharide.In this method,white wax scale polysaccharide was hydrolyzed into component monosaccharides and subsequently derived with 1-phenyl-3-methyl-5-pyrazolone(PMP),the concentration and pH of buffer were studied and optimized.The results showed extracting ratio of polysaccharide was 2.4%,content of polysaccharide was 256 mg·g-1content of protein was 242 mg·g-1,the polysaccharide consisted of glucose,mannose,galactose with the molar ratio of 1∶0.85∶0.93.