Determination of Monosaccharide Compositions and Contents in Polysaccharide of Garlic by HPLC

OBJECTIVE To establish a RP-HPLC method for the determination of the monosaccharide compositions and contents in polysaccharide of garlic.METHODS The garlic polysaccharide were derivatization with 1-phenyl-3-methyl-5-pyrazolone(PMP) after hydrolyzed into monosaccharides with 2 mol·L-1 H2SO4,monosaccharide derivatives were separated on Shimadzu VP-ODS volum(150 mm×4.6 mm,5 μm) with acetonitril-Acetate buffer adjusted to pH 5.5 with Acetate(22:78) as mobile phase and UV detection at 245 nm.RESULTS The content of monosaccharide compositions were measured in polysaccharide of garlic,molar ratio of mannose,rhamnose,glucuronide,galacturonicacid,glucose,galactose,arabinose was 4.31:4.23:4.19:9.13:27.4:4.99:3.81.CONCLUSION The methods is simple,sensitive,accurate and reliable.The method can be applied for the analysis of quality control and monosaccharide composition and molar ratio in polysaccharide of garlic.