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2008•Journal of Yantai UniversityRequires access

Comparison of Inhibiting and Bactericidal Effects of Seven Kinds of Antibiotic On Vibrio anguillarum

Sun Dong-hong

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Abstract

Double dilution method and inhibition zone method are used to determine the minimum inhibitory concentration (MIC) of seven kinds of antibiotic and to compare the inhibiting and bactericidal effects on Vibrio anguillarum. The results show that the MIC of thiamphenicol is mixmum (0.8 μg/mL) and the MIC of gentamicinum is maxmum(6.4 μg/mL).At the concentration of 5 times of MIC, the inhibition zones of seven kinds of antibiotic are: gentamicinum 1.90 cm, norfloxacinum 1.50 cm, streptomycinum 1.43 cm, oxytetracylinnum 1.43 cm, hydrochloric norfloxacinum 1.43 cm, florfenicol 1.40 cm, and thiamphenicol 1.33 cm. It is concluded that vibrio anguillaram is highly sensitive to gentamicinum and norfloxacinum, and middle sensitive to streptomycinum, oxytetracylinnum, hydrochloric norfloxacinum, florfenicol and thiamphenicol. The combination of norfloxacin and thiamphenicol has the best effect.

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What this paper is about

Double dilution method and inhibition zone method are used to determine the minimum inhibitory concentration (MIC) of seven kinds of antibiotic and to compare the inhibiting and bactericidal effects on Vibrio anguillarum. The results show that the MIC of thiamphenicol is mixmum (0.8 μg/mL) and the MIC of gentamicinum is maxmum(6.4 μg/mL).At the concentration of 5 times of MIC, the inhibition zones of seven kinds of antibiotic are: gentamicinum 1.90 cm, norfloxacinum 1.50 cm, streptomycinum 1.43 cm, oxytetracylinnum 1.43 cm, hydrochloric norfloxacinum 1.43 cm, florfenicol 1.40 cm, and thiamphenicol 1.33 cm. It is concluded that vibrio anguillaram is highly sensitive to gentamicinum and norfloxacinum, and middle sensitive to streptomycinum, oxytetracylinnum, hydrochloric norfloxacinum, florfenicol and thiamphenicol. The combination of norfloxacin and thiamphenicol has the best effect.

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Available abstract

Double dilution method and inhibition zone method are used to determine the minimum inhibitory concentration (MIC) of seven kinds of antibiotic and to compare the inhibiting and bactericidal effects on Vibrio anguillarum. The results show that the MIC of thiamphenicol is mixmum (0.8 μg/mL) and the MIC of gentamicinum is maxmum(6.4 μg/mL).At the concentration of 5 times of MIC, the inhibition zones of seven kinds of antibiotic are: gentamicinum 1.90 cm, norfloxacinum 1.50 cm, streptomycinum 1.43 cm, oxytetracylinnum 1.43 cm, hydrochloric norfloxacinum 1.43 cm, florfenicol 1.40 cm, and thiamphenicol 1.33 cm. It is concluded that vibrio anguillaram is highly sensitive to gentamicinum and norfloxacinum, and middle sensitive to streptomycinum, oxytetracylinnum, hydrochloric norfloxacinum, florfenicol and thiamphenicol. The combination of norfloxacin and thiamphenicol has the best effect.

Key concepts: Thiamphenicol, Florfenicol, Minimum inhibitory concentration, Vibrio anguillarum, Norfloxacin, Antibiotics, Minimum bactericidal concentration, Microbiology

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