Biological Characteristics of Rabbit Marrow Stromal Cells in Vitro

Objective To investigate the biological characteristics of rabbit marrow stromal cells(MSC)in vitro and supply bone /cartilage tissue engineering with fundament theory and technologic support.Methods Rabbit bone marrow was harvested from tibias and iliac crests.MSC were separated by centrifuging and cultured in the DMEM medium without any induced factors.The confluent MSC were released and consequently subcultured for 5 passages.The following dates were contiuously measured:growth curve,cell doubling time,confluent rate,cell activity.Results The growth curves and the doubling times of passage 1～3 were similar,and the doubling time of passage 2 was 20h.Both them began decreasing or prolonging from passage 4 gradully.So do the cell activity and confluent rates of passage 4and 5.Conclusion MSC of passage 1～3 proliferated rapidly and grew stably in vitro.The MSC of passage 1～3 may be well used in autogeneous bone/cartilage tissue engineering.