Study of rabbit bone marrow mesenchymal stem cells cultured in vitro

Objective:To study the isolation,culture and proliferation of rabbit bone marrow mesenchymal stem cells (MSC) in vitro,and investigate their biological properties. Methods:MSC were separated from rabbit iliac bone and purified by density gradient centrifugation. MSC was cultured and expanded in flasks. The MSC morphologic features and growth status were observed,the growth curve of cells was drawn and the rate of attachment was measured. Results:The rabbit MSC attached to the surface of plates,exhibiting a fibroblast-like spindle shape. In vitro culture,MSC proliferated quickly to form colonies. MSC exhibited a growth curve of typical S-shape. The rate of attachment was high. Fourteen hours after reinoculating culture,it was more than 90% of the cells at passage 3. Conclusion:The density gradient centrifugation is feasible for separating MSC. The cultivation of MSC in vitro shows that it increases lastingly and has a fast multiplication speed. MSC can be optimal seed cell source for tissue engineering.