Study on the Isolation and Purification Process of Total Flavonoids from Osmanthus fragrans Lour.with Macroporous Absorption Resin

[Objective] To study the isolation and purification process of total flavonoids from Osmanthus fragrans Lour.with macroporous Absorption resin.[Method] Taking the adsorption capacity and recovery of total flavonoids as index,the optimum the isolation and purification process of total flavonoids from Guizhou O.fragrans were respectively investigated by static test and dynamic test with AB-8 macroporous Absorption resin.[Result] pH,eluant,temperature,concentration of column-loading sample,diameter-height ratio,current velocity and quality ratio of total flavonoids and Absorption resin had great effects on the adsorption capacity and recovery of total flavonoids from O.fragrans.The optimum conditions for extracting and purifying total flavonoids in static test were: the AB-8 type macroporous resin was blended with 4 times of pH 4-5sample solution(solid-liquid ratio of 1∶4 g/ml) together and then stirred to make it reach the saturation adsorption;then,the isolated resin whose volume was 9.4 times as high as total flavonoids in sample solution was eluted with 70% ethanol.The optimum conditions in dynamic test were: 17.86 mg/ml the extracts of total flavonoids from O.fragrans were loaded into the column with diameter-height ratio of 1.5∶21.6 at a current velocity of 2-3 ml/min and then eluted with 2-3 BV of water to remove impurity at a current velocity of 3-4 ml/min.As the temperature increased,the adsorption capacity declined but the elution rate to enlarge.[Conclusion] The optimum conditions for extracting and purifying total flavonoids with AB-8 type macroporous resin are selected in this study,which could provide experimental basis for the industrial production of total flavonoids from O.fragrans.