Comparison of Extraction Method in Arabidopsis Thaliana and Garlic Superoxide Dismutase

Thermal denaturation and phosphate buffer methods were applied to initial extraction of superoxide dismutase(SOD) from Arabidopsis thaliana plants,garnish with acetone method and ammonium sulfate classification salting out method were conducted to purify SOD.In result,the thermal denaturation method showed higher SOD recovery rate in extracting SOD comparing to the phosphate buffer method.SOD extracted using the two methods showed no difference in purification process.The conducted experiment concludes a SOD separation method of higher activity and higher purity.