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Research on extraction of SOD and alliinase from garlic by ammonium sulfate salting-out

Huang Xue-song

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Abstract

To extract and preliminarily purify SOD and alliinase from garlic,desired saturation of ammonium sulfate in two-steps salting-out was settled by gradient sedimentation of extract according to enzyme activity and protein content.40% and 90% saturation was defined as the lower and upper point for two-step ammonium sulfate salting-out of the two enzymes.Conclusion showed that SOD and alliinase couldn be extracted and preliminarily purified through two-step salting-out.

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What this paper is about

To extract and preliminarily purify SOD and alliinase from garlic,desired saturation of ammonium sulfate in two-steps salting-out was settled by gradient sedimentation of extract according to enzyme activity and protein content.40% and 90% saturation was defined as the lower and upper point for two-step ammonium sulfate salting-out of the two enzymes.Conclusion showed that SOD and alliinase couldn be extracted and preliminarily purified through two-step salting-out.

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Available abstract

To extract and preliminarily purify SOD and alliinase from garlic,desired saturation of ammonium sulfate in two-steps salting-out was settled by gradient sedimentation of extract according to enzyme activity and protein content.40% and 90% saturation was defined as the lower and upper point for two-step ammonium sulfate salting-out of the two enzymes.Conclusion showed that SOD and alliinase couldn be extracted and preliminarily purified through two-step salting-out.

Key concepts: Salting out, Ammonium sulfate, Chemistry, Salting, Ammonium, Chromatography, Enzyme, Extraction (chemistry)

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