Rification and Characterization of Superoxide Dismutase from Chinese Caterpillar Funfus

Superoxide dismutase(SOPUD) from Chinese caterpillar fungus was separated and purified through ammonium sulfate precipitation and column chromatography with Sephadex G-75 gel filtration and DEAE-52 ion exchange.The purified enzyme showed a single band on PAGE.The specific activity was 17855.73U/mg,the SOD enzyme was 57.3-fold purified,21.8% of the SOD activity was recovered.At the same time,because SOD enzyme easily lost its activity and could not be kept long,so this paper studied and analyzed the function to SOD activity of different densities of sugar and different densities of organic acid,found that the different densities of sugar had not obvious influence to SOD activity,the different densities of organic acid made SOD activity drop,but with the increase of the density of acid,the degree that SOD activity dropped lightened.