Experimental study on the pathophysiological change and mechanism of acute lung injury in rats with severe acute pancreatitis

Objective To observe the pathophysiological change of acute lung injury in rats with severe acute pancreatitis( SAP),and to explore its pathogenesis mechanism. Methods The 240 healthy male SD( Sprague-Dawley) rats from January 2008 to 2009 January were enrolled in the study,these rats were randomly divided into 8 groups according to random digits table,7 observation groups and control group,the observation groups included 7 different time points of SAP,ie.1h group,3h group,6h group,9h group,12 h group,18 h group,24 h group and control group,with 30 rats in each group. The changes of serum amylase,the ratio of lung tissue wet / dry weight,lung myeloperoxidase( MPO) activity and the lungs permeability index were detected and compared among the 8 groups. Results The lung injury score,the ratio of lung tissue wet / dry weight and lung MPO levels in observation groups were gradually increased,as compared with those in control group,moreover,which in 7 time points were significantly higher than those in control group( P 0. 05). The lung permeability index in observation groups was gradually decreased,as compared with that in control group( P 0. 05). The MPO levels in lung tissue were positively correlated to lung injury score( r = 0. 946,P 0. 01),and the ratio of lung tissue wet / dry weight was positively correlated to lung injury score( r = 0. 879,P 0. 01),however,lungs permeability index was negatively correlated to lung injury score( r = 0. 859,P 0. 01). Conclusion The lung injury degree of rats with SAP is gradually increased with prolongation of course of disease,moreover,the MPO levels in lung tissue are continually increased.