2009•Journal of Anhui Traditional Chinese Medical CollegeRequires access

Content Determination of Berberine Hydrochloride in Qianlietong Soft Capsules by High Performance Liquid Chromatography

Wei An

Abstract

Objective: To establish a high performance liquid chromatography(HPLC) method for content determination of berberine hydrochloride in Qianlietong Soft Capsules.Methods: Venusil MP C18(5 μm,4.6 mm×250 mm) column was used for the HPLC determination,and the mobile phase consisted of water(4.0 g/L phosphate and 7.0 g/L triethylamine)-acetonitrile(75∶25) at a flow rate of 1.0 ml/min.The detection wavelength was 265 nm.Results: Berberine hydrochloride had good linear relationship in the range of 0.0557 4～0.278 7 μg/ml(r=0.999 9).The average recovery was 99.67%(RSD=1.11%,n=5).Conclusion: This method is rapid,accurate with good reproducibility,thus it is suitable for quality control of Qianlietong Soft Capsules.

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Key concepts: Berberine hydrochloride, Chromatography, High-performance liquid chromatography, Triethylamine, Berberine, Chemistry, Reproducibility, Acetonitrile