2006Science and Technology of Cereals,oils and FoodsRequires access

Study on the preparation technology of soy peptides by double-enzyme composite hydrolysis

Zhou Li-gen

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Abstract

The preparation technology of soy peptides by double-enzyme composite hydrolysis was studied.Soy protein isolate was produced into soy peptides based on double-enzyme composite hydrolysis and purification technology.The result showed that the best composite enzymatic hydrolysis condition was: the composite enzymes were made up of neutral proteinase and bromelain with the proportion of 3∶1,pH value of 7.0,substrate concentration of 4.0%,hydrolysis time of 9 h;the best decoloration condition was: quantity of activated carbon of 2.0%,temperature of 50℃,pH value of 3.0,adsorption time of 3 h;the ultra-filtration condition was: NF-2 nanometer membrane,pressure of 1.0 mPa.

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The preparation technology of soy peptides by double-enzyme composite hydrolysis was studied.Soy protein isolate was produced into soy peptides based on double-enzyme composite hydrolysis and purification technology.The result showed that the best composite enzymatic hydrolysis condition was: the composite enzymes were made up of neutral proteinase and bromelain with the proportion of 3∶1,pH value of 7.0,substrate concentration of 4.0%,hydrolysis time of 9 h;the best decoloration condition was: quantity of activated carbon of 2.0%,temperature of 50℃,pH value of 3.0,adsorption time of 3 h;the ultra-filtration condition was: NF-2 nanometer membrane,pressure of 1.0 mPa.

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Available abstract

The preparation technology of soy peptides by double-enzyme composite hydrolysis was studied.Soy protein isolate was produced into soy peptides based on double-enzyme composite hydrolysis and purification technology.The result showed that the best composite enzymatic hydrolysis condition was: the composite enzymes were made up of neutral proteinase and bromelain with the proportion of 3∶1,pH value of 7.0,substrate concentration of 4.0%,hydrolysis time of 9 h;the best decoloration condition was: quantity of activated carbon of 2.0%,temperature of 50℃,pH value of 3.0,adsorption time of 3 h;the ultra-filtration condition was: NF-2 nanometer membrane,pressure of 1.0 mPa.

Key concepts: Hydrolysis, Soy protein, Chemistry, Bromelain, Enzymatic hydrolysis, Composite number, Enzyme, Adsorption

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