Preparation of soy peptides by hydrolyzing soy protein with compound enzyme

The preparation of soy peptides was studied by hydrolyzing soy protein with compound enzyme of papain and AS1.398 neutral protease.The results showed that the optimum conditions were as follows:enzyme activity ratio of papain to AS1.398 neutral protease 1∶1,enzyme dosage 12 500 U/g,mass concentration of substrate 100 g/L,pH 7.5,hydrolysis temperature 40℃.Under the optimum conditions,the relationship of hydrolysis time with degree of hydrolysis was researched,and the result indicated that the better hydrolysis time was 4.5 h.