Effect of Micro-Elements on Growth of Isochrysis galbana

The effects of micro-elements,such as manganese,copper,zinc,molybdenum,and cobalt,in the cultural liquid on the growth of Isochrysis galbana are studied in the batch culture.Manganese,copper,zinc,molybdenum and cobalt are defined as essential elements in the culture of Isochrysis galbana and their beneficial concentrations are defined by single factor experiments.The results of orthogonal experiments show that the optimal concentrations in cultural liquid are: Mn 0.08 mmol·m~(-3),Cu 0.07 mmol·m~(-3),Zn 0.0007 mmol·m~(-3),Mo 0.003 mmol·m~(-3),and Co 0.1 mmol·m~(-3).Comparing with the composition of seawater medium M-f/2,the micro-elements concentrations of Cu and Mn in this culture liquid are the same level,while those of Mo,Zn,and Co are 10 times lower,100 times lower,and 10 times higher as that in M-f/2,respectively. When those micro-elements are added in the cultural liquid,the concentration of Isochrysis galbana cell are raised by 14.3%.