2010•Zhongguo laonianxue zazhiRequires access
Effects of PPAR-γ activation on the expressions of TGF-β and CTGF mRNA in rat mesangial cells induced by advanced glycation end products
Yu Xiao
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Abstract
Objective To investigate the role of peroxisome proliferator-activated receptor(PPAR)-γ activation on the expressions of TGF-β and CTGF mRNA in rat mesangial cells extenuation induced by advanced glycation end products (AGEs).Methods Rat mesangial cells were treated with AGE-modified bovine serum albumin or native bovine serum albumin.Normal mesangial cells without any treatments were as control.TGF-β and CTGF mRNA were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR).The expressions of TGF-β and CTGF mRNA in mesangial cell induced by rosiglitazone (0～10 μmol/L) and the inhibitor of PPAR-γ(10 μmol/L) were tested with RT-PCR.Results PPAR-γ activation relieved TGF-β and CTGF mRNA expressing induced by AGEs(0～400 mg/L) in mesangial cells.Conclusions PPAR-γ activation can relieve the expressions of TGF-β and CTGF mRNA in rat mesangial cells induced by AGEs,suggesting that PPAR-γ activation has a kidney protection in diabetic mellitus through ameliorating extracellular matrix accumulation.