Serum Interleukin-1β Expression and Effect of Interleukin-1β on Pathologica Lchange of Lung in Rats with Renal Ischemia Reperfusion

Objective To investigate serum Interleukin-1β(Il-1β) expression and pathological change of lungs in rats with renal ischemia reperfusion.Methods The animal model of renal ischemia reperfusion injury was setup by operation of 45-min bilateral renal pedicle clamping and later reperfusion for 2h、6h、12h、24h、72h on Sprague-Dawley rats.Samples for serum and the left lung of each animal was taken.The serum sample was for analysis of expression of IL-1βusing ELISA,and the lung sample for histologic examination.Results The expression of serum Il-1βand Scr and the lung injury degree score were gradually increased from sham to IR 12h group.The pathological lesions of lungs were most severe at IR 12h.Conclusion The results of this study demonstrate that the expression of serum Il-1βand Scr were increased in rats with renal ischemia reperfusion.The Il-1βwas involved in the process of pathophysiology in lung injury.