Relationship between apoptosis in lung and changes of serum cytokines

Objective To investigate changes of apoptosis in lung and serum cytokines, and to explore the role of them in lung injury after renal ischemia reperfusion. Methods The animal model of renal ischemia reperfusion injury was made by clamping bilateral renal pedicle for 45 minutes. At 2 h, 6 h,12 h,24 h,72 h after ischemia reperfusion,the serum interleukin-1β(IL-1β) and nitric oxide (NO) were detected,the changes of histopathology and apoptosis in lung were observed. Results After ischemia reperfusion,lung apoptosis,serum IL-1β and NO gradually increased. They reached to a peak value at 12 h after ischemia reperfusion. They gradually decreased at 24 h after ischemia reperfusion. The changes of lung injury were consistent to the changes of detected parameters at each time point after ischemia reperfusion. Conclusions IL-1β and NO released after renal ischemia reperfusion may be one of the reasons of apoptosis and injury in lung. Key words: Acute lung injury; Ischemia reperfusion; Apoptosis; Interleukin-1β;  Nitric oxide