Interleukin-1β Leads to Lungs Injury in Rats With Renal Ischemia Reperfusion Injury.

Objective To investigate the expression of serum interleukin-1β(IL-1β) and the apoptosis of lungs in rats with renal ischemia reperfusion(IR).Methods Forty-eight Sprague-Dawley rats were divided into 6 groups randomly,including IR groups(IR 2 h group,IR 6 h group,IR 12 h group,IR 24 h group,IR 72 h group) and the sham control group.The animal models of IR groups were set up by the operation of 45 minutes bilateral renal pedicle clamping and later reperfusion after 2 h,6 h,12 h,24 h,72 h on Sprague-Dawley rats.Samples for serum and the left lung of each animal were taken.The serum sample was for the analysis of the expression of interleukin1β, scr by the method of ELISA,and the lung sample for apoptosis and histological examination.Results The expression levels of serum interleukin-1β,scr and apoptosis positive cells of lungs were gradually increased from the sham control group to IR 12 h group.The number of cells in lung underwent apoptosis was obviously increased in IR 12 h group than that in the sham control group and other IR groups.The pathological lesions of lungs were most severe at IR 12 h group.Conclusion The expression levels of serum interleukin-1β,scr and apoptosis positive cells of lungs in rats with renal ischemia reperfusion are increased.