Analysis of the distribution and antibiotic resistance of pathogenic bacteria from Xiangya Hospital in 2011

Objectives To investigate the distribution and antibiotic resistance of clinical isolates from Xiangya Hospital of Central South University in 2011 and to provide criteria for the rational use of antibiotics by clinics.Methods Isolates were identified and antimicrobial susceptibility testing was carried out using the Vitek-2 automated microorganism identification system.Data were analyzed using WHONET 5.4 software.Results Of 7 272 isolates collected from Xiangya Hospital in 2011,285(28.67%) were Gram-positive bacteria,4 109(56.51%) were Gram-negative bacteria,1078(14.82%) were fungi.Among the Staphylococcus isolates,51.37% were MRSA and 68.16% were MRSCN.No vancomycin,teicoplanin,or linezolid-resistant strains were noted.Enterococcus faecium and Enterococcus faecalis were resistant to vancomycin at a rate of 3.91% and 3.83%,respectively.ESBLs were detected in 78.30% of Escherichia coli and 63.10% of Klebsiella pneumoniae.Enterobacteriaceae remained highly sensitive to carbapenem antibiotics(resistance rate 6.00%),and a few pan-resistant Enterobacteriaceae were detected for the first time.The proportion of pan-resistant strains of Pseudomonas aeruginosa and Acinetobacter baumannii increased significantly in comparison to the proportion in 2010.Conclusion Most clinical isolates from this hospital were Gram-negative bacilli.Clinical isolates had extensive antibiotic resistance.Pan-resistant Enterobacteriaceae and pan-resistant non-fermenting Gram-negative bacteria are increasing.These developments warrant close scrutiny and effective control measures should be taken.