Surveillance of Antibiotic Resistance in Clinical Isolates from Central Hospital of Xiangtan in 2010

Objective To investigate the antibiotic resistance of clinical strains isolated from Central Hospital of Xiangtan to antimicrobial agents commonly used in 2010,and to provide the evidence for rational use of antibiotics in clinical practice.Methods Kirby-Bauer method was used to test the susceptibility of 2,789 clinical isolates to antimicrobial agents.The results were analyzed by WHONET software.Results Of the 2,789 clinical isolates,Gram negative bacilli and Gram positive cocci accounted for 76.6%(2,137/2,789) and 23.4%(652/2,789),respectively.The top six isolates were Klebsiella spp.(17.1%),Acinetobacter spp.(15.2%),Escherichia coli(14.8%),Pseudomonas aeruginosa(13.2%),Staphylococcus aureus(10.3%),and coagulase negative staphylococci(5.6%).In vitro chemosensitivity test revealed that the detection rates of ESBLs-producing E.coli and Klebsiella spp.were 55.2%% and 26.9%,respectively.Carbapenems resistant strains were found in Enterobacteriaceae spp.Pan-resistant strains were identified in 14.2%(52/367) of Pseudomonas aeruginosa isolates and 27.6%(118/425) of Acinetobacter baumannii.The prevalence rates of MRSA and MRCNS were 70.3%(201/287) and 66.7%(104/156),respectively.No Enterococcus isolate was found resistant to vancomycin,teicoplanin or linezolid.The isolation rates of PRSA and PISA were 2.8%(2/72) and 11.1%(8/72),respectively.Beta-lactamase was produced by 10.8% of the Haemophilus influenzae strains.Conclusions The major clinical pathogenic microorganisms in our hospital in 2010 were Gram negative bacilli.The prevalence of multi-drug resistant strains was increased,especially Pan-resistant Pseudomonas aeruginosa and Acinetobacter baumannii isolates.It is important and valuable to periodically investigate bacterial resistance surveillance for rational selection of antimicrobial agents.