The construction of PCR cDNA library of sika deer liver and the isolation of part cDNA encoding complement C3 alpha chain of sika deer

Objective:To construct PCR cDNA library of sika deer (Cervus nippon Temminck) liver, establish gene data base of sika deer Methods:Total RNA was extracted from sika deer liver and transcribed into cDNA Using PCR library synthetic kit, the PCR cDNA library was constructed followed the Protocol provided by Takara Co The individual cDNAs were isolated and sequenced Results:The cDNA library contains cDNA fragments of different lengths A cDNA encoding complement C3 alpha chain of sika deer was isolated from the library and was submitted to Genbank (accession number AF264631) Conclusion:Have successfully constructed the PCR cDNA library of sika deer liver, from which were isolated partial gene encoding complement C3 alpha chain