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2011•Anhui nongye kexueRequires access

Construction of cDNA Library from the Heart of Sika Deer

Baojin Yao

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Abstract

[Objective]To construct a cDNA library from the heart of sika deer.[Method]Total RNA was extracted from the heart of sika deer,and mRNA was purified.Double-stranded cDNA was synthesized,then connected with vector and electrotransformated to competent cells.The number of clones,recombinant rate and the length of insert fragments were checked.[Result]The capacity of the cDNA library was 3.8×106 with a recombinant rate of 100%.The length of insert fragments ranged from 0.5 kb to 2.0 kb,and the average length was about 1.0 kb.[Conclusion] The cDNA library constructed was qualified.

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What this paper is about

[Objective]To construct a cDNA library from the heart of sika deer.[Method]Total RNA was extracted from the heart of sika deer,and mRNA was purified.Double-stranded cDNA was synthesized,then connected with vector and electrotransformated to competent cells.The number of clones,recombinant rate and the length of insert fragments were checked.[Result]The capacity of the cDNA library was 3.8×106 with a recombinant rate of 100%.The length of insert fragments ranged from 0.5 kb to 2.0 kb,and the average length was about 1.0 kb.[Conclusion] The cDNA library constructed was qualified.

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Available abstract

[Objective]To construct a cDNA library from the heart of sika deer.[Method]Total RNA was extracted from the heart of sika deer,and mRNA was purified.Double-stranded cDNA was synthesized,then connected with vector and electrotransformated to competent cells.The number of clones,recombinant rate and the length of insert fragments were checked.[Result]The capacity of the cDNA library was 3.8×106 with a recombinant rate of 100%.The length of insert fragments ranged from 0.5 kb to 2.0 kb,and the average length was about 1.0 kb.[Conclusion] The cDNA library constructed was qualified.

Key concepts: cDNA library, Complementary DNA, Insert (composites), Recombinant DNA, Biology, Molecular biology, RNA, Gene

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