Identification of Three ESTs of Shuangyang Sika Deer

We amplified the sika deer hepatic tissue cDNA library through PCR with HSV1-2 TK specific primer and obtained a cDNA fragment. Another two cDNA fragments of 500*!bp and 1*!000*!bp were also isolated from sika deer blood cDNA through RT PCR primed with a human IL 1β specific primer. These cDNA fragments were cloned into pGEM T Easy plasmid. Sequencing and BLAST search revealed that EST 1 is homologous to α 2 antiplasmin, EST 2 is homologous to aquaporin 4 (AQP 4), and EST 3 is a novel EST of sika deer containing a 267*!bp ORF.