Enzymolysis of Paphia undulate meat for production of micromolecule peptides
Haiwu Rong
Abstract
Haiwu Rong
Abstract
With Paphia undulate as the raw material,its micromolecule peptides compound was prepared by flavourzyme hydrolysis. The optimal conditions for Paphia undulate of second enzymatic hydrolysis with flavourzyme were determined by using response surface methodology.According to the analysis of response surface methodology,the optimal parameters were extraction temperature of 50℃, enzymolysis time of 3.5 h and Flavourzyme(E) and Paphia undulat(S) ratio of 3.1 ∶100.Under conditions metioned above,enzymatic hydrolysis of peptide was 38.15%(mass fraction),which was close to the pedicted value of 39.07%.
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With Paphia undulate as the raw material,its micromolecule peptides compound was prepared by flavourzyme hydrolysis. The optimal conditions for Paphia undulate of second enzymatic hydrolysis with flavourzyme were determined by using response surface methodology.According to the analysis of response surface methodology,the optimal parameters were extraction temperature of 50℃, enzymolysis time of 3.5 h and Flavourzyme(E) and Paphia undulat(S) ratio of 3.1 ∶100.Under conditions metioned above,enzymatic hydrolysis of peptide was 38.15%(mass fraction),which was close to the pedicted value of 39.07%.
Key concepts: Enzymatic hydrolysis, Hydrolysis, Raw material, Extraction (chemistry), Chemistry, Chromatography, Response surface methodology, Biochemistry